Submitting Purified DNA

Samples sent as purified DNA will be sequenced at a price of:

         96-well plates:              $3.50/sample (additional reads $3.50)

                                                  $336/plate (additional reads $336)

         Individual Samples:     $7/sample

 

Preparing Purified DNA

Purified Plasmids:

 The quality of the sequencing reaction depends on the quality of the purified DNA. Most DNA purification kits should provide DNA at a quality sufficient for sequencing.  DNA should be provided at a concentration of 100ng/ul in a volume of 10ul. You can use this Dilution Calculator to help you get the right concentration.

PCR Products:

 The quality of the sequencing reaction depends on the cleanliness of the PCR product. In our lab we use an EXO/SAP clean-up, but other commercially available protocols will work as well.  To avoid any degradation of the ends of the PCR products we recommend cleaning the samples immediately after the PCR reaction.  Purified PCR products should be provided at a concentration of 50ng/ul in a total volume of about 10ul. You can use this Dilution Calculator to help you get the right concentration.

 

Shipping Purified DNA

As long as the samples are free of contaminates, purified DNA does not have to be shipped on ice.  They can be shipped at room temp overnight or via regular mail.  Make sure that the plates are sealed securely to avoid any cross contamination during shipping. Another way to avoid cross contamination is to send the samples frozen (wet or dry ice). Foil tape is better than plastic tape when shipping on ice as it sticks better.

 

Samples should be sent through the carrier of your choice to:

 

Functional Biosciences, Inc.

505 South Rosa Road

Suite 17

Madison, WI 53719

 

 

Enclose a printed copy of your Sample Submission Form.  Please note that samples are not delivered to our facilities on Saturday or Sundays.