Submitting Purified DNA

Samples sent as purified DNA will be sequenced at a price of:

         96-well plates:              $3.50/sample (additional reads $3.50)

                                                  $336/plate (additional reads $336)

         Individual Samples:     $7/sample

 

Preparing Purified DNA

Purified Plasmids:

 The quality of the sequencing reaction depends on the quality of the purified DNA. Most DNA purification kits should provide DNA at a quality sufficient for sequencing.  DNA should be provided at a concentration of 100ng/ul in a volume of 10ul. You can use this Dilution Calculator to help you get the right concentration.

PCR Products:

 The quality of the sequencing reaction depends on the cleanliness of the PCR product. In our lab we use an EXO/SAP clean-up, but other commercially available protocols will work as well.  To avoid any degradation of the ends of the PCR products we recommend cleaning the samples immediately after the PCR reaction.  Purified PCR products should be provided at a concentration of 50ng/ul in a total volume of about 10ul. You can use this Dilution Calculator to help you get the right concentration.

 

Shipping Purified DNA

Individual samples can be shipped at room temp without degradation of results as long as the samples are free of contaminants. Be sure that each sample is tightly sealed. For 1.7 ml tubes, many customers like to wrap the tops in parafilm as an additional safeguard.

For 96-well plates, we recommend the following to prevent leakage during shipment:

• Seal the plates tightly. Strip caps are the most reliable option, just make sure the caps and plate are compatible, forming a tight seal. If you want to use tape pads we recommend that you use a strong aluminum sealing tape, ship on dry ice (see below), and make sure the tape is firmly sealed around each individual well. In our experience, some plastic tapes can lose their seal when shipped on dry ice.
• Freeze the samples and ship on dry ice. Dry ice will keep the samples frozen during shipment, which should guard against any possible leakage. Cold packs don’t offer the same guarantee, especially during the warm summer months. If you’re confident that your plate is well sealed, you can send purified plasmids and purified pcr products at room temperature without degrading the results.
• Pack the plates snugly to minimize shifting or jostling during shipment. You may want to use some paper or foil to fill any extra space in the box.

Upon receipt, we’ll check the seals and well volumes to make sure that no leakage is evident.

 

Functional Biosciences, Inc.

505 South Rosa Road

Suite 17

Madison, WI 53719

 

 

Enclose a printed copy of your Sample Submission Form.  Please note that samples are not delivered to our facilities on Saturday or Sundays.