Functional Biosciences, Inc.

For Genomic DNA we prefer that the concentration is ~20ng/ul in a total volume of 10ul per well. Primers should be provided at 10uM and we need a minimum volume of 225ul of each primer.

The cost for the PCR, PCR clean-up, and the first sequencing reaction is is:

96-well plates: $480/plate (additional reads $336/plate)

Individual Samples: $9.50/sample (additional reads $7/sample)

Individual samples can be shipped at room temp without degradation of results as long as the samples are free of contaminants. Be sure that each sample is tightly sealed. For 1.7 ml tubes, many customers like to wrap the tops in parafilm as an additional safeguard.

For 96-well plates, we recommend the following to prevent leakage during shipment:

Seal the plates tightly. Strip caps are the most reliable option, just make sure the caps and plate are compatible, forming a tight seal. If you want to use tape pads we recommend that you use a strong aluminum sealing tape, ship on dry ice (see below), and make sure the tape is firmly sealed around each individual well. In our experience, some plastic tapes can lose their seal when shipped on dry ice.
Freeze the samples and ship on dry ice. Dry ice will keep the samples frozen during shipment, which should guard against any possible leakage. Cold packs don’t offer the same guarantee, especially during the warm summer months. If you’re confident that your plate is well sealed, you can send purified plasmids and purified pcr products at room temperature without degrading the results.
Pack the plates snugly to minimize shifting or jostling during shipment. You may want to use some paper or foil to fill any extra space in the box.

Upon receipt, we’ll check the seals and well volumes to make sure that no leakage is evident.

Functional Biosciences, Inc.

505 South Rosa Road

Suite 17

Madison, WI 53719

Enclose a printed copy of your sample submission form. Please note that samples are not delivered to our facilities on Saturday or Sundays.